Related Experiment Video
Updated: May 12, 2026

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Published on: May 21, 2015
The change of LTB4 and 5-LO during pregnancy
Feng Jian1, Yuyan Ma, Zhenping Liu
1Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 107#, West Wenhua Road, Jinan, 250012, People's Republic of China.
Purpose:
The changes of leukotriene B4 (LTB4) in the serum and the 5-lipoxygenase (5-LO) in the decidua in different stages of gestation period were investigated.
Methods:
The ELISA double antibody sandwich method was used to detect the concentration of LTB4 in the serum in 21 cases and its levels of the different stages of pregnancy were analyzed. The real-time PCR method was used to detect the mRNA expression level of 5-LO in the decidua tissue of 20 healthy women in early pregnancy and 21 healthy women in third trimester and its levels in the two stages of pregnancy were compared.
Results:
There were significantly different in the concentration of LTB4 in the serum during the four stages of healthy pregnancy. There were also markedly differences in the mRNA expression of 5-LO in the decidua during the two stages of healthy pregnancy. After pregnancy, the concentration of LTB4 in the serum of the subjects in early pregnancy and second trimester decreased, while in third trimester, its level increased. And after pregnancy, its level decreased. The 5-LO mRNA expression in the decidua in third trimester was obviously higher than the early pregnancy group.
Conclusions:
The changes of LTB4 concentration in serum and the mRNA expression level of 5-LO in decidua may play an important role in the success and maintenance of a healthy pregnancy.
More Related Videos
11:08The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
Published on: March 13, 2014
11:44A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
Published on: September 27, 2017
Related Concept Videos
Teratogenicity
Ovarian Cycle
Development of the Oral Microbiota
Development of Human Microbiota
Gonadal and Placental Hormones
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair growth — and...
Transcytosis of IgG
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...