Uptake kinetics and the effect of minoxidil on human keratinocyte cultures

The antihypertensive drug minoxidil (Lonolox) is rapidly taken up by human keratinocytes (HaCaT-cells). After 3 h the uptake is half of its maximum. Specific minoxidil receptors could not be found. High minoxidil concentrations (greater than 100 micrograms/ml) inhibited the incorporation of thymidine into DNA (ED50 = 263 micrograms/ml) and the incorporation of amino acids into proteins (ED50 = 251 micrograms/ml). The inhibition was reversible up to a concentration of 400 micrograms/ml. In the presence of 100 micrograms/ml minoxidil, the cell division remained active for a longer period of time and a higher cell density was achieved as opposed to the parallel control group. A prolongation of the proliferative capability of keratinocytes may be partially responsible for the positive effect of minoxidil on hair growth.