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Citric acid production by Aspergillus niger van. Tieghem MTCC 281 using waste apple pomace as a substrate
Dinesh Kumar1, Rachna Verma, T C Bhalla
1Abhilashi Institute of Life Sciences, Ner Chowk, Mandi, 175 008 India ; Department of Biotechnology, Himachal Pradesh University, Shimla, 171 005 India.
Abstract:
A solid state fermentation process was tried for the production of citric acid from apple pomace left after juice extraction using Aspergillus niger van. Tieghem MTCC 281 spores as inoculum (36.8 × 10(4) spores/100 g of pomace). The yield of citric acid was optimized by varying the amount of methanol (1-5% v/w), temperature (25-35°C) and time of incubation (1-7 days) for fermentation process. Optimum yield of citric acid (4.6 g/100 g of pomace) was recorded with 4% (v/w) methanol after 5 days of incubation at 30°C.
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