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Published on: January 25, 2018
Identification of genetically diverse genotypes for photoperiod insensitivity in soybean using RAPD markers
R K Singh1, V S Bhatia, Sanjeev Yadav
1National Research centre for Soybean, Khandwa Road, Indore, 452 017 India ; Division of Crop Improvement, Indian Institute of Sugarcane Research, Raibarely Road, Lucknow, 226 002 India.
Summary
Soybean photoperiod sensitivity limits cultivation in India. This study used RAPD markers to identify genetically diverse, photoperiod-insensitive soybean varieties, like MACS 330, for broader genetic improvement.
Area of Science:
- Plant genetics
- Molecular markers
- Crop science
Background:
- Soybean cultivation in India is restricted by photoperiod sensitivity in many varieties.
- Identifying photoperiod-insensitive soybean germplasm is crucial for expanding cultivation areas and improving crop adaptability.
- Genetic diversity analysis is essential for understanding and utilizing valuable traits like photoperiod insensitivity.
Purpose of the Study:
- To estimate the genetic diversity of soybean accessions with varying photoperiod sensitivity.
- To identify potential sources of photoperiod insensitivity for broadening the genetic base of soybean.
- To utilize Random Amplified Polymorphic DNA (RAPD) markers for assessing genetic variation.
Main Methods:
- Genetic diversity was assessed in 44 soybean accessions using 25 RAPD primers.
- Amplicon analysis revealed 199 polymorphic bands, with 89.9% polymorphism.
- UPGMA cluster analysis and Mantel's test were employed to interpret genetic relationships.
Main Results:
- An average polymorphism information content of 0.289 was observed.
- Genetic diversity between soybean pairs ranged from 3.9% to 71.6%, averaging 37.7%.
- Two accessions, MACS 330 and 111/2/1939, were identified as highly photoperiod insensitive and genetically distinct.
Conclusions:
- MACS 330 and 111/2/1939 represent valuable genetic resources for breeding photoperiod-insensitive soybean varieties.
- RAPD markers effectively revealed genetic diversity and identified key accessions for trait improvement.
- The findings support strategies to broaden the genetic base of soybean for enhanced adaptability and wider cultivation.
