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Effect of nitrate availability on expression of multi sensor histidine kinase gene in Spirulina platensis PCC 7345
Pradeep Kumar Logeswaran1, Swapnil Srivastav, Krithika Karunakaran
1Centre for biotechnology, Biological Sciences Group, Birla Institute of Technology and Science, Pilani, 333031 Rajasthan India.
Abstract:
Addition of nitrate (NO3 (-)) to a suspension of NO3 (-)-depleted Spirulina platensis PCC 7345 restored back the PSII reaction centre efficiency. Using RAPD/RT-PCR differential display, we have detected the upregulation of a novel gene in filaments starved of nitrogen when enriched with NO3 (-). Semiquantitative RT-PCR clearly shows that NO3 (-) repletion induces the expression of this gene. Its nucleotide sequence is homologous to multi sensor histidine kinase (hstk) from Spirulina platensis NIES-39 and Spirulina maxima CS-328 genes. The open reading frame of this gene encodes a protein of 98 amino acids contains sensory domain of PAS domain.
