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Updated: May 12, 2026
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Usefulness of a Helicobacter pylori stool antigen test for diagnosing H. pylori infected C57BL/6 mice
Dae-In Moon1, Eun-Hye Shin, Hong-Geun Oh
1Center for Animal Resources Development, Wonkwang University, Iksan, Korea. ; Huvet. Inc., Iksan, Korea.
Abstract:
Among several diagnostic tests, a Helicobacter pylori stool antigen (HpSA) test may offer a useful noninvasive method for diagnosing infection without sacrificing animals. In this study, male C57BL/6 mice (n=6) were infected with H. pylori ATCC 49503 (1×10(8) CFU/mouse) by intragastric inoculation three times at 2-day intervals, and H. pylori infected stool specimens were collected 1, 3, 5, 7, 14, 21 days after infection to assess reliability of the HpSA test. Five of six specimens were positive at 5-21 days after infection, and the sensitivity of the HpSA test was 83.33%. The presence of H. pylori infection was confirmed by the rapid urease test and genomic DNA polymerase chain reaction (PCR), and showed the same results as the HpSA. However, the rapid urease test and genomic DNA PCR are invasive tests and require animal sacrifice to detect H. pylori in gastric biopsy samples. We suggest that an HpSA test kit would be useful and effective for monitoring H. pylori in various laboratory animals, as H. pylori can be easily monitored without sacrificing animals.
Insights
The Helicobacter pylori stool antigen (HpSA) test accurately detects H. pylori infection in mice noninvasively. This method allows for reliable monitoring of H. pylori without the need to sacrifice laboratory animals.
Area of Science:
- Veterinary Science
- Microbiology
- Diagnostic Testing
Background:
- Helicobacter pylori infection is a significant concern in laboratory animal research.
- Current diagnostic methods for H. pylori are often invasive, requiring animal sacrifice.
- Noninvasive diagnostic tools are needed for effective and ethical H. pylori monitoring in research settings.
Purpose of the Study:
- To evaluate the reliability of the Helicobacter pylori stool antigen (HpSA) test.
- To assess the HpSA test's efficacy as a noninvasive method for detecting H. pylori infection in mice.
- To compare HpSA test results with traditional invasive diagnostic techniques.
Main Methods:
- Male C57BL/6 mice were infected with H. pylori via intragastric inoculation.
- Stool specimens were collected at various time points post-infection (1-21 days).
- HpSA test was performed on stool samples; results were confirmed using rapid urease test and PCR on gastric biopsy samples.
Main Results:
- The HpSA test demonstrated a sensitivity of 83.33% in detecting H. pylori.
- Positive HpSA results were observed in 5 out of 6 mice from day 5 to day 21 post-infection.
- HpSA test results correlated with findings from invasive rapid urease and PCR tests.
Conclusions:
- The HpSA test is a reliable and noninvasive tool for diagnosing H. pylori infection in laboratory mice.
- This stool antigen test offers an effective alternative to invasive methods, avoiding animal sacrifice.
- HpSA test kits are recommended for routine monitoring of H. pylori in various laboratory animal models.
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