Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same author

Identification of Age-Associated Circulating Proteins and Lipids in 3800 Comorbidity-Enriched Older Adults from Japan-Based Cohorts Using Olink Assays and MRM Mass Spectrometry.

Journal of proteome research·2026
Same author

Genetic regulation across germline and somatic variation on the Y chromosome contributes to type 2 diabetes.

Nature medicine·2026
Same author

Plasma-targeted proteomic and lipidomic profiling of MASLD, MASH, and hepatitis C virus infection.

Clinical proteomics·2026
Same author

A cross-population compendium of gene-environment interactions.

Nature·2026
Same author

Proteogenomics in cerebrospinal fluid and plasma reveals new biological fingerprint of cerebral small vessel disease.

Nature aging·2025

Related Experiment Video

Updated: May 12, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantitative proteomics using mass spectrometry.

Salvatore Sechi1, Yoshiya Oda

  • 1National Institute of Diabetes and Digestive and Kidney Diseases, NIH, DHHS, Bethesda, MD 20892-5460, USA. Salvatore_Sechi@nih.gov

Drug Discovery Today
|April 12, 2013
PubMed
Summary

Stable isotopes in mass spectrometry revolutionize quantitative proteomics. Labeling methods like in vivo, pre-digestion, and post-digestion offer accurate protein quantification and PTM analysis.

More Related Videos

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Related Experiment Videos

Last Updated: May 12, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Quantitative proteomics relies on accurate protein measurement.
  • Stable isotopes serve as effective internal standards in mass spectrometry.
  • Various labeling strategies exist for proteomic analysis.

Purpose of the Study:

  • To review advancements in stable isotope labeling for quantitative proteomics.
  • To discuss different labeling methodologies and their applications.
  • To highlight achievements in protein concentration and PTM quantification.

Main Methods:

  • Stable isotope labeling (in vivo, in vitro pre-digestion, in vitro post-digestion).
  • Mass spectrometry-based quantification.
  • Analysis of post-translational modifications (PTMs).

Main Results:

  • Stable isotope labeling enables a new era in quantitative proteomics.
  • In vivo labeling offers high accuracy for cultured cells.
  • In vitro methods are versatile for various sample types, including clinical samples.
  • Mass spectrometry strategies show significant progress in relative protein quantification and PTM analysis.

Conclusions:

  • Stable isotope labeling is a powerful tool for quantitative proteomics.
  • Current methods allow for accurate protein and PTM quantification.
  • Further technological development is required to fully understand proteome dynamics.