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Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
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Proteomics and NF-κB: an update.

Ellen Niederberger1, Gerd Geisslinger

  • 1Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Klinikum der Goethe-Universität Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.

Expert Review of Proteomics
|April 12, 2013
PubMed
Summary
This summary is machine-generated.

This review highlights proteomic approaches to uncover new functions of the transcription factor Nuclear Factor kappa B (NF-κB). These methods reveal novel roles in disease and identify new NF-κB interaction partners for future research.

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Area of Science:

  • Molecular Biology
  • Immunology
  • Biochemistry

Background:

  • The transcription factor NF-κB plays crucial roles in apoptosis, inflammation, and immunity.
  • While NF-κB signaling is well-studied, its novel functions and interactions in various diseases are continuously being discovered.

Purpose of the Study:

  • To provide an updated review of proteomic approaches used to study NF-κB signaling.
  • To highlight innovative techniques and new hypotheses regarding NF-κB's role in physiological and pathophysiological processes.

Main Methods:

  • Review of recent scientific literature focusing on proteomic studies of NF-κB.
  • Analysis of innovative techniques applied in NF-κB signaling research.

Main Results:

  • Identification of novel NF-κB functions and target proteins through proteomic analysis.
  • Elucidation of new interaction partners and regulators within the NF-κB activation cascade.

Conclusions:

  • Proteomic approaches are essential for discovering new proteins involved in NF-κB signal transduction.
  • Emerging research opens new avenues for understanding NF-κB's role in diverse diseases and developing targeted therapies.