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Updated: May 12, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Separation of inorganic anions on a chemically bonded 18-crown-6 ether stationary phase in capillary ion
Toyohide Takeuchi1, Kimi Tokunaga, Lee Wah Lim
1Department of Chemistry, Faculty of Engineering, Gifu University, Gifu, Japan. take-t@gifu-u.ac.jp
Abstract:
Inorganic anions were separated on a chemically bonded 18-crown-6 ether (18C6E) stationary phase in capillary ion chromatography. In this study 18C6E groups were chemically bonded on silica gel via reaction with 3-glycidyloxypropyltrimethoxysilane, followed by reaction with 2-aminomethyl-18C6E. Analyte anions were separated in the ion-exchange mode based on the fact that the eluent cation was trapped on the 18C6E and it worked as the anion-exchange site. The eluent cation and eluent anion as well as the eluent concentration affected the retention of the analyte anions. Different selectivity was achieved by using an acetonitrile-rich eluent. The present stationary phase was applied for the determination of UV-absorbing anions contained in saliva samples.
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