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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 12, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

[Double-stranded DNA microarray: principal, techniques and applications].

Yan Pan1, Jin-Ke Wang

  • 1The State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China. panyan19880306@sina.com

Yi Chuan = Hereditas
|April 12, 2013
PubMed
Summary
This summary is machine-generated.

Protein binding microarrays (PBMs) analyze DNA-protein interactions. This high-throughput technique determines transcription factor DNA-binding affinity, specificity, and preferences.

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Related Experiment Videos

Last Updated: May 12, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Context:

  • Protein binding microarrays (PBMs), also known as double-stranded DNA (dsDNA) microarrays, enable high-throughput analysis of DNA-protein interactions.
  • This technique immobilizes diverse dsDNA molecules on a solid support to detect binding with DNA-binding proteins, such as transcription factors (TFs).

Purpose:

  • To review the principles, techniques, and applications of dsDNA microarrays for studying TF-DNA interactions.
  • To highlight the utility of PBMs in characterizing TF DNA-binding specificity, profiles, and target genes.

Summary:

  • dsDNA microarrays facilitate the assessment of DNA-binding affinity, specificity, and preference of TFs.
  • Applications include characterizing numerous TFs, building TF-binding profiles, identifying TF-binding sites and target genes, discriminating TF family member preferences, and examining cofactor effects.

Impact:

  • PBMs offer a powerful tool for understanding gene regulation by TFs.
  • This technology accelerates the characterization of TF functions and their roles in biological processes.
  • It aids in identifying potential therapeutic targets by revealing TF-mediated gene expression patterns.