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The host factor RAD51 is involved in mungbean yellow mosaic India virus (MYMIV) DNA replication

Geetika Suyal1, Sunil K Mukherjee, Nirupam R Choudhury

  • 1Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi, 110067, India. gits27@gmail.com

Archives of Virology
|April 12, 2013
PubMed

Insights

This study reveals that the host factor AtRAD51 interacts with the mungbean yellow mosaic India virus (MYMIV) Rep protein, aiding in viral DNA replication. This finding highlights a new mechanism in begomovirus replication.

Area of Science:

  • Plant virology
  • Molecular biology
  • Biochemistry

Background:

  • Geminiviruses, including mungbean yellow mosaic India virus (MYMIV), utilize host factors for replication via rolling-circle replication (RCR) and recombination-dependent replication.
  • The viral Rep protein is crucial for initiating and regulating geminivirus DNA replication.

Purpose of the Study:

  • To investigate the interaction between MYMIV Rep protein and the host factor AtRAD51.
  • To determine the role of AtRAD51 in MYMIV replication.

Main Methods:

  • Yeast two-hybrid and β-galactosidase assays to demonstrate protein-protein interaction.
  • Co-immunoprecipitation assay to confirm the interaction.
  • Yeast-based geminivirus DNA replication restoration assay to assess functional complementation.
  • Semiquantitative RT-PCR and northern hybridization to analyze gene expression.

Main Results:

  • Direct interaction between MYMIV Rep and AtRAD51 was confirmed using multiple assays.
  • AtRAD51 functionally complemented a yeast rad51 mutation in a geminivirus replication assay.
  • Rad51 transcript levels were significantly higher in MYMIV-infected mungbean plants compared to healthy controls.

Conclusions:

  • Eukaryotic RAD51 protein is involved in MYMIV replication, suggesting a cross-talk between RAD51 and MYMIV Rep.
  • This interaction likely plays a role in controlling viral DNA replication in plants.
  • The findings provide insights into the molecular machinery governing begomovirus DNA replication.

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