Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Purification and characterization of arginase from a new Aspergillus niger AUMC16187 isolate from sandy soil.

Archives of microbiology·2026
Same author

Editorial: Unraveling circRNAs and miRNAs: key regulators in immune-related diseases.

Frontiers in immunology·2025
Same author

Investigating the role of bulk and nano nickel in amelioration of morphophysiology and photosynthetic activity of Triticum aestivum L.

Scientific reports·2025
Same author

Unveiling Talaromyces marneffei emergence among HIV/AIDS patients: exploring phylogeny and molecular identification.

Cellular and molecular biology (Noisy-le-Grand, France)·2025
Same author

Parkinson's disease detection from EEG signal employing autoencoder and RBFNN-based hybrid deep learning framework utilizing power spectral density.

Digital health·2024
Same author

Monoclonal Antibody Delivery Using 3D Printed Biobased Hollow μNe3dle Arrays for the Treatment of Osteoporosis.

Molecular pharmaceutics·2024

Related Experiment Video

Updated: May 12, 2026

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

Sponge-like: a new protocol for preparing bacterial ghosts.

Amro A Amara1, Mounir M Salem-Bekhit, Fars K Alanazi

  • 1Department of Protein Research, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt. amroamara@web.de

Thescientificworldjournal
|April 12, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for producing bacterial ghosts (BGs) using sub-inhibitory concentrations of chemical agents. The developed protocol yields high-quality BGs suitable for diverse biotechnological applications.

More Related Videos

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii
09:04

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii

Published on: April 16, 2016

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Related Experiment Videos

Last Updated: May 12, 2026

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii
09:04

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii

Published on: April 16, 2016

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Area of Science:

  • Biotechnology
  • Microbiology
  • Biochemistry

Background:

  • Bacterial ghosts (BGs) are increasingly recognized for their potential in medicine and pharmaceuticals.
  • Existing methods for BG production can be complex or inefficient.

Purpose of the Study:

  • To develop and optimize a novel, generalizable protocol for bacterial ghost production.
  • To establish conditions for high-quality bacterial ghost (BG) preparation using E. coli BL21 (DE3) pLysS.

Main Methods:

  • Utilized sub-minimum inhibitory concentrations (MIC) of SDS, NaOH, and H2O2 for BG production.
  • Employed Plackett-Burman experimental design to determine optimal production conditions.
  • Assessed bacterial ghost quality (BGQ) using light and electron microscopy, and quantified protein/DNA release via spectrophotometry.

Main Results:

  • Successfully established a new protocol for bacterial ghost production.
  • Optimized chemical treatment concentrations and conditions for efficient BG generation.
  • Verified the quality and purity of produced bacterial ghosts, with minimal residual DNA.

Conclusions:

  • The developed protocol provides a reliable method for producing high-quality bacterial ghosts.
  • These bacterial ghosts are suitable for various biotechnological and pharmaceutical applications.
  • This method offers a promising advancement in bacterial ghost production technology.