Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Dialysis01:15

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On hidden and visible charges in mass spectrometry: alternative approaches in mass spectral interpretation.

Analytical methods : advancing methods and applications·2026
Same author

The place of analytical chemistry in clinical pharmacology.

British journal of clinical pharmacology·2026
Same author

A McLafferty-Type Rearrangement in the Pentafluorobenzyl Ester Derivatives of Dialkyl Phosphates in GC-NICI-MS/MS: A Mini-Review and a Meta-Analysis.

Molecules (Basel, Switzerland)·2026
Same author

Quantitative GC-MS/MS in the clinical analysis of eicosanoids: A critical review and discussion, a 40-years historical retrospect, and possible implications for LC-MS/MS.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

The significance of endocannabinoid receptors and endocannabinoids in the lower urinary and genital tract.

World journal of urology·2025
Same author

Refreshing the Aims and Scope of the Journal of Chromatography B.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2025

Related Experiment Video

Updated: May 12, 2026

Custom-made Microdialysis Probe Design
05:38

Custom-made Microdialysis Probe Design

Published on: July 21, 2015

Enhanced human tissue microdialysis using hydroxypropyl-ß-cyclodextrin as molecular carrier.

Marcus May1, Sandor Batkai, Alexander A Zoerner

  • 1Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany.

Plos One
|April 12, 2013
PubMed
Summary

Hydroxypropyl-ß-cyclodextrin (HP-ß-CD) enhances microdialysis recovery of lipophilic molecules in human tissues. This method improves measurement of interstitial anandamide and other challenging molecules without adverse effects.

More Related Videos

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
08:17

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Related Experiment Videos

Last Updated: May 12, 2026

Custom-made Microdialysis Probe Design
05:38

Custom-made Microdialysis Probe Design

Published on: July 21, 2015

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
08:17

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Area of Science:

  • Biomedical Engineering
  • Pharmacology
  • Analytical Chemistry

Background:

  • Microdialysis struggles with lipophilic molecules due to protein binding and membrane adhesion, limiting recovery.
  • Hydroxypropyl-ß-cyclodextrin (HP-ß-CD) is known to improve recovery of hydrophobic molecules in vitro and in animal models.

Purpose of the Study:

  • To evaluate the efficacy and safety of HP-ß-CD as a microdialysis perfusion fluid supplement in human subjects.
  • To determine the impact of HP-ß-CD on metabolite stability, delivery, and recovery in human adipose tissue and muscle.

Main Methods:

  • In vitro assessment of HP-ß-CD effects on metabolite stability and recovery.
  • Microdialysis catheter insertion into subcutaneous adipose tissue and vastus lateralis muscle of 20 healthy volunteers.
  • Perfusion with Ringer solution with or without 10% HP-ß-CD at varying flow rates (0.3-2.0 µl/min).
  • Analysis of tissue metabolites, ultrafiltration, and blood flow.

Main Results:

  • Metabolite concentrations in dialysate were equal or higher when using HP-ß-CD.
  • Addition of HP-ß-CD increased dialysate volume by 10%.
  • No adverse local or systemic reactions were observed; analytical methods remained unaffected.
  • HP-ß-CD enabled the measurement of interstitial anandamide, a highly lipophilic molecule.

Conclusions:

  • HP-ß-CD is a safe and effective supplement for clinical microdialysis in humans.
  • This approach significantly enhances the recovery of lipophilic molecules from human interstitial fluid.
  • HP-ß-CD facilitates the measurement of previously challenging lipophilic analytes in vivo.