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Related Experiment Videos

Cold-sensitive responses in the Paramecium membrane.

T Inoue1, Y Nakaoka

  • 1Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.

Cell Structure and Function
|April 1, 1990
PubMed
Summary

Paramecium cells exhibit temperature-sensitive depolarization when cooled. Cooling rate influences depolarization amplitude, suggesting sensitivity to temperature change rate and altered membrane conductances.

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Area of Science:

  • Cellular electrophysiology
  • Membrane biophysics
  • Single-cell analysis

Background:

  • Deciliated Paramecium exhibits transient depolarization upon cooling.
  • Depolarization amplitude correlates with cooling rate, indicating sensitivity to temperature change.
  • Membrane input resistance increases during cooling.

Purpose of the Study:

  • Investigate Paramecium's electrophysiological response to cooling.
  • Determine the relationship between cooling rate and depolarization amplitude.
  • Analyze the influence of ion concentrations on temperature-induced membrane potential changes.

Main Methods:

  • Recording of transient depolarization in deciliated Paramecium during cooling.
  • Application of constant current to alter resting membrane potential.
  • Manipulation of extracellular potassium (K+) and calcium (Ca2+) concentrations.

Main Results:

  • Depolarization amplitude is proportional to cooling rate.
  • Cooling causes a transient increase in membrane input resistance.
  • Reversal potential of cooling response is independent of K+ but slightly dependent on Ca2+.
  • Initial depolarization amplitude decreases with increased K+ and is unaffected by Ca2+.

Conclusions:

  • Paramecium's membrane exhibits sensitivity to the rate of temperature change.
  • Cooling induces changes in membrane conductances, affecting ion permeability.
  • Electrophysiological responses to cooling are modulated by extracellular ion concentrations, particularly Ca2+.

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