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Published on: July 13, 2011
Induction of 5'-deiodinase activity in rat astroglial cells by acidic fibroblast growth factor
F Courtin1, J M Gavaret, D Toru-Delbauffe
1Unité de Recherche sur la Glande Thyroïde et la Régulation Hormonale, Institut National de la Santé et de la Recherche Médicale, Kremlin-Bicêtre, France.
Abstract:
Acidic fibroblast growth factor (aFGF) induced a large increase in the type II 5'-deiodinase (5'D) activity in astroglial cells. This required a time lag of about 4 h. Half-maximal stimulation was obtained with about 7 ng/ml aFGF. This factor at 20 ng/ml induced several times more 5'D activity than did 20 ng/ml basic fibroblast growth factor (bFGF) after 8 h incubation. aFGF (20 ng/ml) produced a 10-50-fold increase in 5'D activity after 24 h, whereas the effect of 20 ng/ml bFGF had disappeared after 24 h. Heparin (17 micrograms/ml) potentiated the 5'D response to natural and recombinant aFGF. Glucocorticoids amplified the aFGF-induction of 5'D activity. This is the first demonstration in astroglial cells that a growth factor can regulate the 5'D activity.
