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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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High sensitive gold-nanoparticle based lateral flow Immunodevice for Cd2+ detection in drinking waters.

Adaris M López Marzo1, Josefina Pons, Diane A Blake

  • 1Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology, Campus de la UAB, 08193 Bellaterra, Barcelona, Spain.

Biosensors & Bioelectronics
|April 13, 2013
PubMed
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A new lateral flow immunosensor device (LFID) detects cadmium (Cd2+) in water with unprecedented sensitivity. This paper-based sensor achieves the lowest detection limit for metal detection, significantly below drinking water standards.

Area of Science:

  • Analytical Chemistry
  • Biosensors
  • Environmental Science

Background:

  • Accurate and sensitive detection of heavy metals like cadmium in water is crucial for public health.
  • Existing methods for cadmium detection can be complex, time-consuming, and require specialized equipment.
  • Lateral flow devices (LFDs) offer potential for rapid, on-site water quality monitoring.

Purpose of the Study:

  • To introduce a novel lateral flow immunosensor device (LFID) for the determination of cadmium ions (Cd2+) in drinking and tap water.
  • To develop a highly sensitive and specific method for cadmium detection using a competitive assay format.
  • To optimize the LFID for enhanced sensitivity and reduced detection limits.

Main Methods:

  • Development of a Cd-EDTA-BSA-AuNP conjugate as a signal producer.

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  • Utilizing a competitive reaction between the conjugate and sample Cd-EDTA for antibody binding sites.
  • Immobilization of a Cd-EDTA specific monoclonal antibody (2A81G5) on the test line.
  • Optimization of reagent concentrations to enhance sensitivity in the LFD.

    • Main Results:

    • The LFID demonstrated a broad response range (0.4–2000 ppb) with a linear response between 0.4 and 10 ppb.
    • Achieved quantification and detection limits of 0.4 and 0.1 ppb, respectively, the lowest reported for paper-based metal sensors.
    • The detection limit is 50 times lower than the maximum contamination level for drinking water.
    • Demonstrated effective masking of metal interferences using optimized EDTA and OVA concentrations.

    Conclusions:

    • The developed LFID provides a highly sensitive and rapid method for cadmium detection in water.
    • The sensor's low detection limit meets stringent drinking water quality standards.
    • The study presents a novel strategy for enhancing sensitivity in competitive format LFDs.
    • The device shows good specificity, making it suitable for real-world water quality monitoring.