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Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
James A Casbon1, Andrew F Slatter, Esther Musgrave-Brown
1Population Genetics Technologies Ltd., Babraham Institute, Babraham, Cambridgeshire CB22 3AT, UK.
Abstract:
We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.
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