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Updated: May 12, 2026
08:34
Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
Published on: September 28, 2022
Summary
Researchers purified CD146+ pericytes from human tissues. These pericytes support the self-renewal and proliferation of transplantable human cord blood hematopoietic stem cells (HSCs).
Area of Science:
- Hematology
- Stem Cell Biology
- Cell Biology
Background:
- Hematopoietic stem cells (HSCs) are crucial for blood formation and immune reconstitution.
- Understanding the niche that supports HSC self-renewal and proliferation is vital for regenerative medicine.
- Pericytes are perivascular cells with diverse functions, including roles in stem cell niches.
Purpose of the Study:
- To investigate the potential of CD146+ pericytes as supportive cells for human cord blood hematopoietic stem cells (HSCs).
Main Methods:
- Purification of CD146+ pericytes from human adipose and fetal bone marrow.
- Co-culture of purified pericytes with human cord blood HSCs.
- Assessment of HSC self-renewal and proliferation capacity.
Main Results:
- CD146+ pericytes isolated from human adipose or fetal bone marrow support the self-renewal of transplantable human cord blood HSCs.
- These pericytes also enhance the proliferation of human cord blood HSCs.
Conclusions:
- CD146+ pericytes represent a promising cell type for supporting HSC function.
- This finding has implications for improving HSC transplantation and regenerative medicine strategies.
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