Different colony formation in low- and high-responding strains of mice

B lymphocytes from the immunized low-responding strain B10 did not form colonies after stimulation with SRBC in semisolid agar cultures, this defect was reparable by simultaneous stimulation with LPS and SRBC. The colony formation of macrophages obtained from control and primarily immunized mice was comparable, but after secondary immunization the B10-derived macrophages formed only 50% of colonies. The formation of haemopoietic CFU-S colonies is in B10 strain significantly suppressed by both primary and secondary immunization.