Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On the applicability of x-ray strain imaging using the edge illumination technique in biomedical applications.

Journal of physics D: Applied physics·2025
Same author

The cool brown dwarf Gliese 229 B is a close binary.

Nature·2024
Same author

T staging esophageal tumors with x rays.

Optica·2024
Same author

Framework to optimize fixed-length micro-CT systems for propagation-based phase-contrast imaging.

Optics express·2024
Same author

A dynamical measure of the black hole mass in a quasar 11 billion years ago.

Nature·2024
Same author

On the equivalence of the X-ray scattering retrieval with beam tracking and analyser-based imaging using a synchrotron source.

Journal of physics D: Applied physics·2023

Related Experiment Video

Updated: May 12, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

X-ray phase-contrast imaging with nanoradian angular resolution.

P C Diemoz1, M Endrizzi, C E Zapata

  • 1Department of Medical Physics and Bioengineering, UCL, WC1E 6BT London, United Kingdom.

Physical Review Letters
|April 16, 2013
PubMed
Summary
This summary is machine-generated.

A novel x-ray imaging technique achieves nanoradian sensitivity using edge illumination, enabling high-resolution imaging across diverse experimental conditions for scientific breakthroughs.

More Related Videos

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Related Experiment Videos

Last Updated: May 12, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Area of Science:

  • Physics
  • Materials Science
  • Biomedical Imaging

Background:

  • Quantitative X-ray Phase-Contrast Imaging (XPCi) is crucial for high-resolution imaging.
  • Existing XPCi methods face limitations in sensitivity and applicability across energy ranges.

Purpose of the Study:

  • To introduce a new quantitative XPCi method with unprecedented nanoradian sensitivity.
  • To demonstrate the method's efficacy across a broad range of experimental conditions and X-ray energies.

Main Methods:

  • Development of a novel quantitative X-ray phase-contrast imaging technique.
  • Utilizing the edge illumination principle for enhanced sensitivity.
  • Experimental validation at two synchrotron radiation facilities.

Main Results:

  • Achieved unprecedented nanoradian sensitivity in X-ray imaging.
  • Demonstrated high angular resolution theoretically and experimentally.
  • Confirmed efficient performance across very high and very low X-ray energies.

Conclusions:

  • The developed XPCi method offers exceptional sensitivity and broad applicability.
  • This technique has the potential to unlock new scientific applications in biology, medicine, and materials science.