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Published on: June 14, 2016
MUC5AC expression correlates with invasiveness and progression of extramammary Paget's disease
1Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Background:
Patients with in situ extramammary Paget's disease (EMPD) tend to have a good prognosis, although dermal invasion and metastasis are associated with significantly increased morbidity and mortality. Previous studies have addressed mechanisms underlying the EMPD pathogenesis; however, no molecular markers that reflect invasiveness or progression have been established.
Objective:
This study aims to identify a reliable marker for predicting the risk of invasion and metastasis in EMPD.
Methods:
We performed an initial microarray screening for in situ, invasive or metastatic lymph node lesions of EMPD. We analysed 44 specimens from 38 primary EMPD cases by immunohistochemical staining.
Results:
We found that expressions of MUC5AC directly correlate with invasion and prognosis. Labelling rates of tumour cells were scored by staining intensity on a four-tiered scale (- to 3+) to investigate the correlation between the expression score of these molecular markers and the type of EMPD lesion. All the specimens scored positive (3+) for MUC1 and negative (-) for MUC6. MUC5AC expression was detected in 19 of 44 (43.2%) specimens. Invasive lesions and metastatic lymph nodes tended to express MUC5AC significantly higher than in situ lesions (P < 0.01). MUC2 was positive in 10 specimens (22.7%). There was no significant difference between the degree of MUC2 expression and invasiveness.
Conclusion:
The degree of MUC5AC expression may correlate with the invasiveness and progression of EMPD, and may be a useful marker for identifying high-risk EMPD cases.
Insights
MUC5AC expression may predict extramammary Paget
Area of Science:
- Oncology
- Molecular Pathology
- Cancer Biomarkers
Background:
- Extramammary Paget's disease (EMPD) prognosis varies; dermal invasion and metastasis increase mortality.
- Current molecular markers for EMPD invasiveness and progression are lacking.
Purpose of the Study:
- Identify a reliable marker to predict invasion and metastasis risk in EMPD.
- Establish a prognostic indicator for high-risk EMPD cases.
Main Methods:
- Microarray screening of in situ, invasive, and metastatic EMPD lesions.
- Immunohistochemical staining of 44 specimens from 38 primary EMPD cases.
- Scoring MUC5AC expression intensity on a four-tiered scale.
Main Results:
- MUC5AC expression correlated significantly with invasion and prognosis in EMPD.
- Invasive and metastatic EMPD lesions showed higher MUC5AC expression than in situ lesions (P < 0.01).
- MUC1 was universally positive (3+), MUC6 was negative, and MUC2 showed no significant correlation with invasiveness.
Conclusions:
- MUC5AC expression levels may indicate EMPD invasiveness and progression.
- MUC5AC shows potential as a valuable biomarker for identifying high-risk EMPD.
- Further validation of MUC5AC as a prognostic marker is warranted.
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