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Updated: May 12, 2026
11:34
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Published on: May 5, 2014
Detection of RNA viruses: current technologies and future perspectives
Lakshmi N Cella1, Daniel Blackstock, Marylynn A Yates
1Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware 19716, USA. clakshmi@udel.edu
Critical Reviews in Eukaryotic Gene Expression
|April 16, 2013
Summary
This review covers viral detection technologies, highlighting nucleic acid-based methods as superior for sensitive and rapid identification of RNA viruses. These advanced techniques are poised to become the standard for diagnosing viral infections.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- RNA viruses are significant human pathogens.
- Accurate detection is crucial for managing viral diseases.
- Traditional methods like cell culture are time-consuming.
Purpose of the Study:
- To review conventional and emerging technologies for RNA virus detection.
- To compare the efficacy and advancements in viral detection methods.
- To highlight the shift towards molecular diagnostic approaches.
Main Methods:
- Review of cell culture-based viral detection.
- Analysis of nucleic acid-based techniques including PCR and microarrays.
- Exploration of emerging methods for detecting RNA in live cells.
Main Results:
- Cell culture remains a gold standard but is being superseded.
- Nucleic acid-based technologies offer high sensitivity, selectivity, and speed.
- Multiplex assays and microarrays enable simultaneous detection of multiple viruses.
Conclusions:
- Nucleic acid-based technologies are revolutionizing viral detection.
- Standardization of these molecular methods will lead to widespread adoption.
- Cell culture may be limited to highly specific applications in the future.
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