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Related Concept Videos

Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Lineage Commitment01:21

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Commitment is the  process whereby stem cells:
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Stem Cell Niche01:26

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
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Maintenance of the ES Cell State01:14

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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
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Related Experiment Video

Updated: Apr 30, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
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Guards at the gate to embryonic stem cell differentiation.

Norihiro Tsuneyoshi1, N Ray Dunn

  • 1Institute of Medical Biology, A(∗)STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, Singapore 138648, Singapore.

Cell
|April 16, 2013
PubMed
Summary

This study reveals unexpected genetic players involved in flipping the switch from pluripotency to differentiation. Understanding these factors is key to controlling cell fate decisions.

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A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • The core gene network maintaining pluripotency is partially understood.
  • The precise mechanisms initiating differentiation remain largely undefined.

Discussion:

  • This study investigates the genetic triggers for cellular differentiation.
  • Unexpected genes influencing the pluripotency-to-differentiation transition were identified.

Key Insights:

  • Identified novel genetic regulators of cell fate.
  • Revealed previously unrecognized factors in differentiation pathways.

Outlook:

  • Further research into these unexpected players could unlock new therapeutic strategies.
  • This work advances our understanding of fundamental developmental processes.