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Published on: December 24, 2014
Solubilization of phenanthrene above cloud point of Brij 30: a new application in biodegradation
T Pantsyrnaya1, S Delaunay, J L Goergen
1Laboratoire Réactions et Génie des Procédés UPR CNRS 3349, University of Lorraine, ENSAIA - 2, Avenue de la Forêt de Haye, 54505 Vandoeuvre-les-Nancy Cedex, France. tatiana.v.p@hotmail.com
Abstract:
In the present study a new application of solubilization of phenanthrene above cloud point of Brij 30 in biodegradation was developed. It was shown that a temporal solubilization of phenanthrene above cloud point of Brij 30 (5wt%) permitted to obtain a stable increase of the solubility of phenanthrene even when the temperature was decreased to culture conditions of used microorganism Pseudomonas putida (28°C). A higher initial concentration of soluble phenanthrene was obtained after the cloud point treatment: 200 against 120μM without treatment. All soluble phenanthrene was metabolized and a higher final concentration of its major metabolite - 1-hydroxy-2-naphthoic acid - (160 against 85μM) was measured in the culture medium in the case of a preliminary cloud point treatment. Therefore a temporary solubilization at cloud point might have a perspective application in the enhancement of biodegradation of polycyclic aromatic hydrocarbons.
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