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Related Experiment Video

Updated: May 12, 2026

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Pan-myeloid Differentiation of Human Cord Blood Derived CD34⁺ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

Human mast cells arise from a common circulating progenitor.

Katariina Maaninka¹, Jani Lappalainen, Petri T Kovanen

¹Wihuri Research Institute, Helsinki, Finland.

The Journal of Allergy and Clinical Immunology

|April 16, 2013

Summary

Human mast cells (MCs) originate from a common progenitor. All circulating MC progenitors can differentiate into MCs expressing a full panel of neutral granule proteases, revealing MC heterogeneity.

Keywords:

CPA3 Carboxypeptidase A3 Chymase KITLG Kit ligand MC MC(T)MC(TC)MITF Mast cell Mast cell containing tryptase Mast cell containing tryptase and chymase Microphthalmia transcription factor kit ligand mast cell neutral granule protease tryptase

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Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

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Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

Area of Science:

Immunology
Cell Biology
Hematology

Background:

Human mast cells (MCs) exhibit phenotypic heterogeneity marked by neutral granule proteases.
Previous understanding limited MC phenotypes to tryptase-only or tryptase/chymase expressing cells.
The developmental relationship between MCs with different protease profiles remained unclear.

Purpose of the Study:

To investigate the relationship between human mast cells (MCs) with distinct protease phenotypes and their circulating progenitors.
To elucidate the differentiation potential of MC progenitors.

Main Methods:

Human peripheral blood CD34(+) progenitors were cultured with kit ligand (KITLG) and cytokines (IL-3, IL-9, IL-6) or KITLG alone.
Expression of key proteases (chymase, CPA3, cathepsin G, granzyme B, tryptases) was assessed at mRNA and protein levels.

Main Results:

Culture with KITLG and cytokines generated a uniform MC population expressing tryptase, chymase, CPA3, cathepsin G, and granzyme B.
KITLG alone was sufficient to induce the expression of all tested proteases in differentiating MCs.

Conclusions:

All circulating human MC progenitors can differentiate into MCs expressing a complete set of neutral granule proteases.
This suggests a common MC-committed progenitor for all human MC phenotypes.