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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 12, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Label-free cell profiling.

Richard B M Schasfoort¹, Arthur E H Bentlage, Ivan Stojanovic

¹Medical Cell Biophysics Group, MIRA Institute, University of Twente, Enschede, The Netherlands. r.b.m.schasfoort@utwente.nl

Analytical Biochemistry

|April 16, 2013

Summary

This summary is machine-generated.

This study presents a rapid, label-free method using surface plasmon resonance (SPR) imaging for red blood cell (RBC) profiling. The technique efficiently identifies RBC surface antigens in under six minutes, enabling detailed cell characterization.

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Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

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Last Updated: May 12, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

Area of Science:

Biomedical Engineering
Analytical Chemistry
Cell Biology

Background:

Accurate cell profiling is crucial for diagnostics and research.
Label-free detection methods reduce experimental complexity and cost.
Red blood cell (RBC) membrane antigen analysis is important for transfusion medicine.

Purpose of the Study:

To develop and validate a rapid, label-free method for red blood cell (RBC) profiling using surface plasmon resonance (SPR) array imaging.
To demonstrate the capability of the SPR method for identifying RBC surface antigens.

Main Methods:

Utilized surface plasmon resonance (SPR) array imaging for label-free cell analysis.
Employed a flow chamber with a spotted sensor surface containing antibodies against RBC membrane antigens.
Analyzed sensorgrams to identify cell sedimentation and specific cell-antigen binding events.

Main Results:

Developed a complete RBC profiling cycle in under 6 minutes.
Demonstrated specific binding of RBCs to immobilized antibodies on the SPR sensor.
Confirmed sensor surface regenerability for at least 100 cycles, enabling repeated analysis.

Conclusions:

The SPR array imaging method provides a fast and effective platform for label-free RBC profiling.
This technique allows for the determination of RBC surface antigen profiles.
The method's efficiency and reusability offer significant advantages for cell analysis.