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Construction, expression, and purification of recombinant αVβ5 integrin

Lawrence J Tartaglia1, Antonette Bennett, Andrew G Woodhouse

  • 1Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL 32610, United States.

Protein Expression and Purification
|April 16, 2013
PubMed
Summary

Researchers developed a novel recombinant integrin expression system for large-scale production of alphaVbeta5 integrin extracellular domains. This efficient method yields sufficient quantities for structural and functional studies.

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