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Related Experiment Video

Updated: May 12, 2026

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

Electrochemical As(III) whole-cell based biochip sensor.

Fernando Cortés-Salazar¹, Siham Beggah, Jan Roelof van der Meer

¹Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Station 6, CH-1015 Lausanne, Switzerland.

Biosensors & Bioelectronics

|April 16, 2013

Summary

A novel whole-cell biosensor using engineered Escherichia coli detects arsenite in water. This electrochemical biochip offers a simple, rapid, and sensitive method for arsenic detection, crucial for safe drinking water.

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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Area of Science:

Environmental Science
Biotechnology
Electrochemistry

Background:

Arsenite poses a significant threat to public health, necessitating sensitive detection methods.
Escherichia coli possesses natural resistance mechanisms against toxic arsenic species.
Existing arsenic detection methods can be complex and time-consuming.

Purpose of the Study:

To develop a whole-cell based electrochemical biosensor for arsenite detection.
To leverage engineered Escherichia coli for sensitive and rapid arsenic analysis.
To create a portable and user-friendly device for water quality monitoring.

Main Methods:

Engineered Escherichia coli with a genetic circuit linking arsenite detection to beta-galactosidase synthesis.
Integration of the reporter strain into a microchip with 16 electrochemical cells.
Electrochemical signal generation for arsenite quantification.

Main Results:

The biosensor demonstrated a limit of detection of 0.8 ppb in tap water.
Achieved excellent linear responses in tested concentration ranges (R(2) values of 0.9975 and 0.9991).
Results from tap and groundwater samples showed strong agreement with atomic absorption spectroscopy.

Conclusions:

The developed electrochemical biochip provides a simple, fast, and sensitive method for arsenite detection.
The biosensor complies with World Health Organization limits for arsenic in drinking water.
This assay is a viable alternative for portable quantification of arsenic (III) in water samples.