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Growing Magnetotactic Bacteria of the Genus Magnetospirillum: Strains MSR-1, AMB-1 and MS-1
Published on: October 17, 2018
Lysinibacillus manganicus sp. nov., isolated from manganese mining soil
Hongliang Liu1, Yumei Song1, Fang Chen1
1State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Abstract:
A Gram-stain-positive, aerobic, motile, rod-shaped bacterium, designated strain Mn1-7(T), was isolated from manganese mining soil in Tianjin, China. The closest phylogenetic relatives were Lysinibacillus massiliensis CCUG 49529(T) (97.2 % 16S rRNA gene sequence similarity), L. xylanilyticus XDB9(T) (96.7 %), L. sinduriensis JCM 15800(T) (96.2 %), L. odysseyi NBRC 100172(T) (95.9 %) and L. boronitolerans NBRC 103108(T) (95.4 %) (the type species of the genus). DNA-DNA hybridization values for strain Mn1-7(T) with the type strains of L. massiliensis and L. sinduriensis were 24.9 and 27.7 %, respectively. The genomic DNA G+C content was 38.4 mol%. The major menaquinone was MK-7 and the major fatty acids were iso-C15 : 0, iso-C16 : 0 and iso-C14 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was type A4α (L-Lys-D-Asp), and the predominant cell-wall sugar was xylose. DNA-DNA hybridization results and comparison of phenotypic and chemotaxonomic characters between strain Mn1-7(T) and the phylogenetically most closely related strains revealed that the isolate represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus manganicus sp. nov. is proposed. The type strain is Mn1-7(T) ( = DSM 26584(T) = CCTCC AB 2012916(T)).
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