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Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
Published on: September 19, 2016
Dietary L-proline supplementation confers immunostimulatory effects on inactivated Pasteurella multocida vaccine
Wenkai Ren1, Lingxiu Zou, Zheng Ruan
1Key Laboratory of Agroecology and Processing of Subtropical Region, Institute of Subtropical Agriculture, Research Center for Healthy Breeding of Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central China, The Chinese Academy of Science, Ministry of Agriculture, 410125, Changsha City, Hunan, People's Republic of China.
Abstract:
This study was conducted to determine the immunostimulatory effect of L-proline on inactivated vaccine immunized mice. Ninety-five female KM mice were randomly divided into five groups: (1) mice received dietary supplementation with 0.4% L-proline and immunized with inactivated vaccine (V-P group); (2) mice received dietary supplementation with 0.3% L-alanine (isonitrogenous control) and immunized with inactivated vaccine (V-A group, negative control); (3) mice were immunized with inactivated vaccine with oil adjuvant (V-O group, positive control); (4) mice were immunized with inactivated vaccine with aluminum hydroxide adjuvant (V-H group, positive control); (5) mice immunized with phosphate-buffered saline (control group). All mice were dead in the control group between 36 and 48 h post infection. Mice in the V-P group showed 100% protection after challenge with P. multocida serotype A (CQ2) at dose of 4.4 × 10(5) CFU (2LD50). Meanwhile, serum antibody titers in the V-P group were higher than those in the V-A group before infection and those in the V-A and V-O groups at 36 h post infection. Moreover, serum IL-1β levels in the V-P group were lower than those in V-O group. Furthermore, serum GSH-PX levels in the V-P group were higher than those in the V-A and V-O groups. Collectively, dietary proline supplementation confers beneficial immunostimulatory effects in inactivated P. multocida vaccine immunized mice.
