Related Experiment Video
Updated: May 12, 2026

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
Published on: June 2, 2014
Human olfactory receptors: recombinant expression in the baculovirus/Sf9 insect cell system, functional
Valéry Matarazzo1, Catherine Ronin
1Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille, CRN2M, CNRS UMR6231, Université Paul Cézanne, Marseille, France.
Abstract:
Cell surface expression of recombinant olfactory receptors (ORs) is a major limitation in characterizing their functional nature. We have shown that the recombinant expression of a human OR, OR 17-210, in the baculovirus/Sf9 insect cell system allows this protein to be expressed at the cell surface. We used Ca(2+) imaging to demonstrate that recombinant OR 17-210 produces cellular activities upon odorant stimulation with ketones. Furthermore, this expression and functional system has been used to show that the preincubation of Human Odorant Binding Protein 2A decrease the calcium response of OR 17-210 following stimulation by acetophenone and beta ionone.
Related Concept Videos
Olfactory Receptors: Location and Structure
Olfaction
The olfactory receptors are embedded in the cilia of the...
Physiology of Smell and Olfactory Pathway
The olfactory...
