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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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Published on: January 18, 2017

Fluorescence microscopy-a historical and technical perspective.

¹Department of Obstetrics & Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York 10461; Eunice Kennedy Shriver Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.

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|April 16, 2013

Summary

This summary is machine-generated.

Fluorescence microscopy has driven cell biology discoveries for over 100 years. This review details its principles and evolution, aiding researchers in selecting imaging methods for specific scientific questions.

Keywords:

diffraction-limited microscopy fluorescence microscopy historical perspective super-resolution microscopy

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Area of Science:

Cell Biology
Microscopy
Biotechnology

Background:

Fluorescence microscopy has been pivotal in cell biology for over a century.
Technological advancements have significantly enhanced imaging and quantification capabilities.
A robust methodology now underpins fluorescence microscopy techniques.

Observation:

This work outlines the fundamental principles of fluorescence microscopy.
It traces the historical development of the technique.
The review covers the expanding array of available tools.

Findings:

Provides insight into the evolution of fluorescence microscopy.
Offers a comprehensive overview of current methodologies.
Highlights the advancements in visualization and quantification.

Implications:

Aids researchers in understanding the expanding toolkit of fluorescence microscopy.
Facilitates the selection of appropriate fluorescence microscopy methods for specific research objectives.
Supports informed decision-making in experimental design for cell biology studies.