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Updated: May 12, 2026

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Published on: March 5, 2019

Vascular calcifying progenitor cells possess bidirectional differentiation potentials.

Hyun-Ju Cho¹, Hyun-Jai Cho, Ho-Jae Lee

¹National Research Laboratory for Stem Cell Niche, Seoul National University College of Medicine, Seoul, Korea.

|April 16, 2013

Summary

Vascular calcification, a feature of atherosclerosis, may be targeted by specific bone marrow-derived progenitor cells. Activating peroxisome proliferator activated receptor γ (PPARγ) in these cells may reverse calcification.

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Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

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Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Published on: April 16, 2021

Area of Science:

Vascular Biology
Stem Cell Biology
Atherosclerosis Research

Background:

Vascular calcification is a hallmark of atherosclerosis with limited therapeutic options.
Identifying progenitor cells involved in vascular calcification is crucial for developing new treatments.

Purpose of the Study:

To identify and characterize vascular calcifying progenitor cells.
To investigate their potential for modulating or reversing vascular calcification.

Main Methods:

Mouse aortic cells were sorted using Sca-1 and PDGFRα markers.
Progenitor cell differentiation potential was assessed in vitro.
In vivo ectopic and atherosclerotic calcification models were used to evaluate therapeutic potential.
Peroxisome proliferator activated receptor γ (PPARγ) agonist treatment was applied.

Main Results:

Sca-1(+) progenitor cells showed higher osteoblastic potential than Sca-1(-) cells.
Sca-1(+)/PDGFRα(-) cells exhibited bidirectional differentiation, while Sca-1(+)/PDGFRα(+) cells differentiated unidirectionally.
PPARγ activation in Sca-1(+)/PDGFRα(-) cells promoted osteoclast-like differentiation and reduced calcification in vivo.
Treatment with PPARγ agonist significantly decreased atherosclerotic plaque severity.

Conclusions:

Bone marrow-derived, vessel-resident Sca-1(+) progenitor cells are implicated in vascular calcification.
PPARγ activation in Sca-1(+)/PDGFRα(-) cells presents a potential therapeutic strategy for reversing vascular calcification.