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Related Concept Videos

Primary Active Transport01:29

Primary Active Transport

In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would not...
Primary Active Transport01:47

Primary Active Transport

In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they...
Resting Potential Decay01:15

Resting Potential Decay

The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane through...
Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: May 12, 2026

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
06:43

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

Synchronization modulation increases transepithelial potentials in MDCK monolayers through Na/K pumps.

Vu Tran1, Xiaodong Zhang, Lin Cao

  • 1Institute for Regenerative Cures, Departments of Dermatology and Ophthalmology, University of California Davis, Davis, California, United States of America.

Plos One
|April 16, 2013
PubMed
Summary
This summary is machine-generated.

Synchronization modulation (SM) electrically enhances transepithelial potential (TEP) by optimizing Na/K pump activity. This novel technique shows potential for improving wound healing and managing diseases involving TEP.

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Area of Science:

  • Physiology
  • Biophysics
  • Cell Biology

Background:

  • Transepithelial potential (TEP) is crucial for ion transport in polarized epithelia.
  • Epithelial injury disrupts TEP, creating endogenous electric fields that guide cell migration for wound healing.
  • Enhancing TEP is a potential strategy to improve wound healing.

Purpose of the Study:

  • To develop and evaluate a novel electrical technique to enhance TEP.
  • To investigate the underlying mechanisms of TEP modulation.
  • To explore potential applications in wound healing and disease management.

Main Methods:

  • Developed synchronization modulation (SM), an electrical pulse train technique.
  • Applied SM to kidney epithelial monolayers (MDCK cells).
  • Assessed TEP changes and the role of Na/K pump activity and distribution.

Main Results:

  • SM significantly increased TEP by over fourfold.
  • SM-induced TEP increases were abolished by Na/K pump inhibitors (ouabain, digoxin).
  • Basolateral Na/K pump distribution was essential for SM efficacy.

Conclusions:

  • Synchronization modulation is a novel electrical approach to significantly increase TEP.
  • The technique targets and enhances Na/K pump activity.
  • This method holds promise for modulating TEP in wound healing and related diseases.