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Updated: May 12, 2026

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
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Fluorogenic Enzyme-Responsive Micellar Nanoparticles.

Miao-Ping Chien1, Matthew P Thompson, Eugene C Lin

  • 1Department of Chemistry & Biochemistry, University of California, San Diego, La Jolla, CA, U.S.A.

Chemical Science
|April 16, 2013
PubMed
Summary
This summary is machine-generated.

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Researchers developed enzyme-responsive fluorogenic micelles using peptide-polymer amphiphiles. These nanoparticles enable detection of enzymatic activity and analysis of micelle stability and structure through FRET signals.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Micellar nanoparticles offer versatile platforms for sensing applications.
  • Enzyme-responsive materials are crucial for targeted drug delivery and diagnostics.
  • Fluorescence resonance energy transfer (FRET) is a powerful tool for studying molecular interactions and assembly.

Purpose of the Study:

  • To develop novel enzyme-responsive fluorogenic micellar nanoparticles.
  • To utilize FRET for detecting enzymatic activity and assessing nanoparticle properties.
  • To elucidate the internal structure and stability of the assembled micelles.

Main Methods:

  • Assembly of micelles from fluorescein and rhodamine-labeled peptide-polymer amphiphiles (PPAs).
  • Utilizing Förster Resonance Energy Transfer (FRET) between dye pairs to monitor particle formation.

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A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
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A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

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Last Updated: May 12, 2026

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

  • Spectrophotometric analysis to determine critical aggregation concentration and intraparticle distances.

    • Main Results:

    • Demonstrated successful assembly of fluorogenic micelles from PPAs.
    • Showcased FRET signal generation indicative of particle assembly and enzymatic activity.
    • Quantified micelle stability and determined amphiphile packing within the micelles.

    Conclusions:

    • Enzyme-responsive fluorogenic micelles provide a sensitive platform for detecting enzymatic activity.
    • FRET-based analysis enables characterization of nanoparticle stability and internal structure.
    • These findings contribute to the development of advanced nanomaterials for diagnostics and sensing.