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Related Concept Videos

Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...

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Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood
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Addressing endotoxin issues in bioengineered heparin.

Jiraporn Suwan1, Amanda Torelli, Akihiro Onishi

  • 1Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.

Biotechnology and Applied Biochemistry
|April 17, 2013
PubMed
Summary
This summary is machine-generated.

Bioengineered heparin production faces challenges with endotoxin removal. This study assesses current methods and strategies to ensure safety for preclinical evaluation of this vital anticoagulant.

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Last Updated: May 12, 2026

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07:48

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Published on: May 16, 2016

Area of Science:

  • Biotechnology
  • Pharmaceutical Science
  • Biochemistry

Background:

  • Heparin, a critical anticoagulant, is traditionally sourced from porcine intestines.
  • A 2008 contamination event highlighted risks associated with animal-derived pharmaceuticals.
  • Bioengineered heparin offers a potential alternative, produced via bacterial fermentation and processing.

Purpose of the Study:

  • To investigate challenges in reducing or removing endotoxins during bioengineered heparin production.
  • To ensure the safety of bioengineered heparin for preclinical in vivo evaluation.
  • To assess and improve the endotoxin removal strategies for the manufacturing process.

Main Methods:

  • Assessing endotoxin levels in the current bioengineered heparin production process.
  • Evaluating strategies for endotoxin removal from intermediate polysaccharides.
  • Examining methods for endotoxin reduction associated with enzymatic processing steps.

Main Results:

  • The current bioengineered heparin process harbors significant endotoxin contamination.
  • Specific purification strategies are being explored to mitigate endotoxin levels.
  • Further refinement is needed to meet safety standards for in vivo studies.

Conclusions:

  • Endotoxin contamination is a key hurdle in developing safe, bioengineered heparin.
  • Targeted endotoxin removal strategies are crucial for advancing this therapeutic.
  • Successful implementation will enable preclinical testing of this novel anticoagulant.