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Updated: May 12, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
Published on: September 2, 2025
Plasmids as scribbling pads for operon formation and propagation
Vic Norris1, Annabelle Merieau
1Theoretical Biology Unit, Department of Biology, University of Rouen, 76821 Mont Saint Aignan cedex, France. Victor.Norris@univ-rouen.fr
Abstract:
Many bacterial genes are in operons and the process whereby operons are formed is therefore fundamental. To help elucidate this process, we propose in the Scribbling Pad hypothesis that bacteria have been constantly using plasmids for genetic experimentation and, in particular, for the construction of operons. This hypothesis simultaneously solves the problems of the creation of operons and the way operons are propagated. We cite results in the literature to support the hypothesis and make experimental predictions to test it.
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