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Related Concept Videos

RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: May 12, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

RNA helicases involved in U-insertion/deletion-type RNA editing.

Elisabeth Kruse1, Christin Voigt, W-Matthias Leeder

  • 1Darmstadt University of Technology, Darmstadt, Germany.

Biochimica Et Biophysica Acta
|April 17, 2013
PubMed
Summary

RNA editing in African trypanosomes involves U nucleotide insertion/deletion guided by guide RNAs (gRNAs) within the editosome. This review focuses on editing RNA helicases, crucial for this unique biological process.

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A Nonsequencing Approach for the Rapid Detection of RNA Editing
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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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Published on: July 22, 2014

Area of Science:

  • Molecular Biology
  • Parasitology
  • Biochemistry

Background:

  • Kinetoplastid protozoa, like African trypanosomes, possess a unique mitochondrial RNA editing system.
  • This process modifies pre-messenger RNAs (pre-mRNAs) by inserting/deleting uridines (U), converting them into functional transcripts.
  • RNA editing is mediated by the editosome, a large protein complex involving guide RNAs (gRNAs) and accessory proteins.

Purpose of the Study:

  • To review the structural, genetic, and biochemical insights into two characterized editing RNA helicases.
  • To provide an outlook on the dynamic processes involved in the RNA editing reaction cycle.

Main Methods:

  • Literature review of structural, genetic, and biochemical data.
  • Analysis of the roles of accessory proteins, including RNA helicases, in RNA editing.

Main Results:

  • RNA editing is a complex pathway involving the editosome, gRNAs, and accessory factors like RNA helicases.
  • Specific RNA helicases of the DEAD- and DExH/D-box families are key players in facilitating RNA editing reactions.

Conclusions:

  • Editing RNA helicases are essential components of the editosome, contributing to the dynamic steps of RNA editing.
  • Understanding these helicases offers insights into the regulation and mechanism of this vital protozoan process.