Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Retinal neurotransmitter interaction as reflected in horizontal cell spatial behaviour.

B D Drujan1, R Salas, M Laufer

  • 1Laboratory of Neurophysiology, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.

Journal of Neural Transmission. Supplementum
|January 1, 1990
PubMed
Summary

The study reveals that 5-hydroxytryptamine (5-HT) and its precursors, along with dopamine (DA), alter fish retinal horizontal cell properties by causing cell uncoupling. This process involves indolaminergic cells triggering DA release from interplexiform cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a comprehensive real-world evidence dataset to inform diabetes policy: the diabetes data cell project.

BMC public health·2026
Same author

Impact of pregestational obesity on perinatal complications: update in a Latin American cohort.

Public health·2024
Same author

Breeding density affects the movements of gull chicks, the size of their home ranges and their association with neighbours.

Royal Society open science·2024
Same author

Val/Met BDNF as a genetic risk for a false sense of security in post-discharge suicide risk.

Journal of affective disorders·2024
Same author

Corrigendum to "SELNET clinical practice guidelines for soft tissue sarcoma and GIST" [Cancer Treat. Rev. 102 (2021) 102312].

Cancer treatment reviews·2023
Same author

Decreased Brain Ventricular Volume in Psychiatric Inpatients with Serotonin Reuptake Inhibitor Treatment.

Chronic stress (Thousand Oaks, Calif.)·2022

Area of Science:

  • Neuroscience
  • Retinal Physiology
  • Cellular Signaling

Background:

  • Horizontal cells play a crucial role in visual processing within the retina.
  • Serotonin (5-HT) and dopamine (DA) are known neurotransmitters influencing retinal function.
  • The interplay between these neurotransmitters and horizontal cell spatial properties requires further elucidation.

Purpose of the Study:

  • To investigate the effects of 5-hydroxytryptamine (5-HT) and its precursors (5-HTP, L-Tryp) on the spatial properties of fish retinal horizontal cells.
  • To compare the efficacy of 5-HT and its precursors with dopamine (DA) in modulating horizontal cell function.
  • To elucidate the underlying cellular mechanisms, including cell coupling and neurotransmitter release.

Main Methods:

  • Experiments were conducted on isolated and perfused retinas of the teleost Eugerres plumieri.

Related Experiment Videos

  • Receptive field properties were assessed by comparing responses to local and distant light stimuli.
  • Cell uncoupling was evaluated using intracellular injection of Lucifer yellow.
  • High-performance liquid chromatography (HPLC) was used to measure endogenous DA release.
  • Pharmacological agents like Haloperidol were employed to block specific pathways.

    • Main Results:

    • 5-HT, 5-HTP, and L-Tryp induced a contraction of the horizontal cell receptive field, indicating cell uncoupling.
    • Dopamine exhibited a stronger uncoupling effect than 5-HT, which was more potent than 5-HTP and L-Tryp (DA > 5-HT > 5-HTP > L-Tryp).
    • Haloperidol blocked all observed effects, suggesting a dopaminergic mechanism.
    • 5-HT and 5-HTP stimulated endogenous DA release from retinal interplexiform cells, while L-Tryp did not.

    Conclusions:

    • Indolaminergic cells (producing 5-HT) stimulate the release of DA from interplexiform cells in the fish retina.
    • This DA release subsequently leads to the uncoupling of horizontal cells, altering their spatial properties.
    • The findings reveal a novel pathway for neurotransmitter-mediated regulation of retinal circuitry.