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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Proteomic data show an increase in autoantibodies and alpha-fetoprotein and a decrease in apolipoprotein A-II with
Summary
Senescence-accelerated mice (SAMP8) show altered serum protein levels, including increased autoantibodies and alpha-fetoprotein, and decreased apolipoprotein A-II, starting at two months of age. These changes may indicate early aging biomarkers.
Area of Science:
- Gerontology
- Biochemistry
- Immunology
Background:
- Senescence-accelerated mouse-prone 8 (SAMP8) mice exhibit accelerated aging phenotypes.
- Understanding age-related proteomic changes is crucial for identifying biomarkers of aging.
Purpose of the Study:
- To investigate age-related changes in serum protein profiles of SAMP8 mice compared to age-matched SAMR1 controls.
- To identify potential protein biomarkers for early aging.
Main Methods:
- Serum samples from SAMP8 and SAMR1 mice at various ages (2, 6, 12, 15 months) were analyzed using 2D electrophoresis and mass spectrometry.
- Protein alterations were quantified and identified.
Main Results:
- Seven altered protein spots were identified in SAMP8 serum, including Ig kappa chain V region (M-T413), alpha-fetoprotein, and apolipoprotein A-II.
- M-T413 RNA levels were elevated in SAMP8 splenocytes, correlating with lower CD4+ T cell counts.
- Increased autoantibodies and alpha-fetoprotein, and decreased apolipoprotein A-II were observed in SAMP8 mice from 2 months onwards.
Conclusions:
- Serum protein profiles in SAMP8 mice show significant age-related alterations.
- Alpha-fetoprotein, autoantibodies, and apolipoprotein A-II are potential biomarkers for early aging in SAMP8 mice.
