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06:38
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Published on: December 8, 2023
Genome-based bacterial vaccines: current state and future outlook.
Alexandra Schubert-Unkmeir1, Myron Christodoulides
1Institute of Hygiene and Microbiology, University of Wuerzburg, Josef-Schneider-Straße 2, 97080, Wuerzburg, Germany, aunkmeir@hygiene.uni-wuerzburg.de.
Summary
Genome-based reverse vaccinology (RV) identifies vaccine candidates from pathogen genomes using bioinformatics. This strategy, exemplified by Bexsero, is advancing new vaccines against bacterial and eukaryotic pathogens.
Area of Science:
- Genomics
- Vaccinology
- Bioinformatics
Background:
- Genome-based reverse vaccinology (RV) utilizes in silico analysis of whole genome sequences to identify potential vaccine antigens.
- The first RV-developed vaccine, Bexsero®, targets Neisseria meningitidis serogroup B.
- RV is expanding to combat emerging bacterial diseases and eukaryotic pathogens.
Purpose of the Study:
- To review the current status of genome-based RV-engineered vaccines.
- To explore future applications of RV in vaccinology.
- To highlight emerging structure-based vaccinology approaches.
Main Methods:
- In silico analysis of whole genome sequences.
- Application of bioinformatic algorithms for antigen identification.
- Integration of structural biology data in structure-based vaccinology.
Main Results:
- Successful development of Bexsero® for Neisseria meningitidis serogroup B prevention.
- Ongoing RV application for novel vaccines against challenging bacterial infections.
- RV strategy extended to develop vaccines against helminths, protozoa, and ectoparasites.
Conclusions:
- Genome-based RV is a powerful platform for vaccine discovery and development.
- Continued advancements in sequencing and bioinformatics will enhance RV efficacy.
- Empirical validation remains crucial for selecting optimal antigen combinations for effective vaccines.
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