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Comparison of total serum lipids measured by two methods
J E Heubi1, R J Sokol, C A McGraw
1Division of Pediatric Gastroenterology and Nutrition, Children's Hospital Research Foundation, Cincinnati, Ohio.
Summary
Accurate vitamin E status assessment requires measuring serum vitamin E and total lipids. Two methods for total lipids showed excellent correlation, with the measured method preferred for clinical use due to cost and ease.
Area of Science:
- Clinical Chemistry
- Nutritional Biochemistry
Background:
- Accurate assessment of vitamin E status is crucial for patient health.
- The ratio of serum vitamin E to total serum lipids is a key indicator.
- Reliable measurement of total serum lipids is essential for this assessment.
Purpose of the Study:
- To compare two distinct methods for measuring total serum lipids.
- To evaluate the clinical applicability of these methods for vitamin E status assessment.
Main Methods:
- Comparison of a calculated lipid sum (cholesterol, triglycerides, phospholipids) against a colorimetric method.
- Analysis of total lipid concentrations ranging from 151-1,728 mg/dl.
Main Results:
- Excellent correlation (r = 0.930) was observed between the two lipid measurement methods across the tested range.
- No significant differences were found between 0-1,000 mg/dl, but the colorimetric method yielded higher values above 1,000 mg/dl (p = 0.023).
Conclusions:
- Both methods are comparable for clinical assessment of vitamin E/total lipid status.
- The measured total lipids method is preferred due to its lower cost and simpler execution.