Related Experiment Video
Updated: May 12, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve
Stefan Jezierski1, Anke S Klein, Christian Benz
1Institut für Analytische Chemie, Universität Leipzig, Johannisallee 29, 04103 Leipzig, Germany.
Abstract:
We immobilized adherent human embryonic kidney (HEK) cells--which are able to trace adenosine triphosphate (ATP)--inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.
More Related Videos
Related Concept Videos
Capillary Electrophoresis: Instrumentation
Overview Of Cell Separation And Isolation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview
There...
Microbial Biosensors

