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Three-dimensional structural analysis of MgO-supported osmium clusters by electron microscopy with single-atom

Ceren Aydin1, Apoorva Kulkarni, Miaofang Chi

  • 1Department of Chemical Engineering and Materials Science, University of California, One Shields Avenue, Davis, CA 95616, USA.

Angewandte Chemie (International Ed. in English)
|April 18, 2013
PubMed
Summary

No abstract available in PubMed .

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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