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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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A Phox2b::FLPo transgenic mouse line suitable for intersectional genetics.

Marie-Rose Hirsch1, Fabien d'Autréaux, Susan M Dymecki

  • 1Institut de Biologie de l'Ecole normale supérieure (IBENS), CNRS UMR8197, INSERM U1024, 75005, Paris, France.

Genesis (New York, N.Y. : 2000)
|April 18, 2013
PubMed
Summary

A new mouse model, P2b::FLPo, enables precise tracking of Phox2b-expressing neurons crucial for autonomic functions. This tool overcomes developmental study limitations by permanently labeling these cells for research.

Keywords:
BAC transgenic mouse lineFLP recombinasePhox2bfate mappinghindbrain

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Phox2b is a key transcription factor for developing neurons controlling vital autonomic functions.
  • Studying Phox2b-expressing neuronal populations is challenging due to cell migration and lack of specific markers.

Purpose of the Study:

  • To develop a novel genetic tool for lineage tracing of Phox2b-expressing neurons.
  • To overcome limitations in studying the development of visceral function-regulating neurons.

Main Methods:

  • Generation of a BAC transgenic mouse line, P2b::FLPo, expressing FLP recombinase in Phox2b-positive cells.
  • Utilizing FLP-responsive alleles (RC::Fela) for permanent lineage tracing.
  • Combining P2b::FLPo with Cre drivers (e.g., Lbx1(Cre)) for intersectional fate mapping.

Main Results:

  • The P2b::FLPo line successfully labels cells expressing or having expressed Phox2b via FLP recombination.
  • This system allows permanent marking and tracking of these neuronal populations throughout development.
  • Intersectional labeling with Lbx1(Cre) enables specific isolation of double-positive neuronal subsets.

Conclusions:

  • The P2b::FLPo mouse line is a valuable tool for dissecting the development and function of Phox2b-dependent neuronal circuits.
  • This technology facilitates genetic access to specific neuronal subsets previously inaccessible.
  • Enables advanced research into the development of cardiovascular, respiratory, and digestive systems.