Related Experiment Videos
In vitro alterations in human fibroblast behavior secondary to silicone polymers
R L McCauley1, W B Riley, R A Juliano
1Division of Plastic Surgery, University of Texas Medical Branch, Galveston.
The Journal of Surgical Research
|July 1, 1990
Summary
Silicone gel in prostheses alters human fibroblast behavior, reducing cell proliferation and increasing endoplasmic reticulum. This suggests silicone polymers significantly impact cellular responses, potentially contributing to fibrous capsular contractures.
Area of Science:
- Biomaterials Science
- Cell Biology
- Tissue Engineering
Background:
- Fibrous capsular contractures are a complication associated with silicone prostheses.
- The exact causes of these contractures remain incompletely understood.
- Cellular reactions to silicone biomaterials have not been extensively investigated.
Purpose of the Study:
- To investigate the cellular effects of silicone gel components on human dermal fibroblasts.
- To explore the potential mechanisms by which silicone prostheses may influence fibroblast behavior.
Main Methods:
- Exposure of human dermal fibroblasts to silicone gel components.
- Assessment of cellular configuration changes.
- Quantification of cell proliferation using matrix protein assays and hemocytometer counts.
- Analysis of cellular ultrastructure via transmission electron microscopy.
Main Results:
- Exposure to silicone gel induced significant changes in fibroblast morphology.
- Cell proliferation was progressively reduced in fibroblasts exposed to silicone gel.
- Transmission electron microscopy revealed a twofold increase in rough endoplasmic reticulum in treated cells.
Conclusions:
- Silicone polymers in prostheses induce significant alterations in human fibroblast behavior.
- Changes in fibroblast proliferation and endoplasmic reticulum suggest a cellular response to silicone components.
- These findings may offer insights into the etiology of fibrous capsular contractures.