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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Published on: October 27, 2013
A new freshwater amoeba: Cochliopodium pentatrifurcatum n. sp. (Amoebozoa, Amorphea)
Yonas I Tekle1, Anderson O Roger, Ariel F Lecky
1Department of Biology, Spelman College, 350 Spelman Lane Southwest, Atlanta, Gerogia 30314, USA. ytekle@spelman.edu
Abstract:
Cochliopodium pentatrifurcatum n. sp. (ATCC(©) 30935(TM) ) is described based on light microscopic morphology, fine structure, and molecular genetic evidence. Cochliopodium pentatrifurcatum n. sp. (length ~ 25 μm) is characterized by surface microscales (0.3 μm tall) containing a circular porous base (~ 0.6 μm diam.) with a thin peripheral rim. Five radially arranged feet, emanating from the base, support a short central column terminating apically as a funnel-shaped collar (~ 0.5 μm diam.) composed of five radial, trifurcate rays extending from the center toward a thin peripheral rim. The central spine is 0.5-0.6 μm long. The comparative morphologies and combined molecular genetic evidence, SSU-rDNA and COI, indicate that the new species falls in a clade sufficiently different from other species to suggest that it is a valid new species.
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