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Updated: May 12, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

Specific serology for emerging human coronaviruses by protein microarray.

C Reusken¹, H Mou, G J Godeke

¹Centre for Infectious Disease Control, Division Virology, National Institute for Public Health and the Environment, Bilthoven, The Netherlands. chantal.reusken@rivm.nl

Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin

|April 19, 2013

Summary

This summary is machine-generated.

A new protein microarray assay detects IgM and IgG antibodies for human coronavirus EMC (hCoV-EMC) and SARS-CoV. This validated method distinguishes between emerging and known coronaviruses using spike protein antigens.

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Area of Science:

Immunology
Virology
Biotechnology

Background:

Emerging human coronaviruses (hCoVs) pose significant public health threats.
Accurate serological assays are crucial for diagnosing and understanding coronavirus infections.
Distinguishing between different hCoV infections, including SARS-CoV and hCoV-EMC, is clinically important.

Purpose of the Study:

To develop and validate a specific serological assay for detecting IgM and IgG antibodies against hCoV-EMC and SARS-CoV.
To utilize protein microarray technology for sensitive and specific viral antibody detection.
To differentiate antibodies against emerging hCoV-EMC from those against SARS-CoV and common hCoVs.

Main Methods:

Development of a protein microarray assay employing the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens.
Extensive validation of the assay using serum samples.
Testing of sera from patients exposed to common hCoVs to assess cross-reactivity.
Testing of sera from convalescent patients infected with hCoV-EMC or SARS-CoV to confirm specificity.

Main Results:

The protein microarray assay demonstrated specific detection of IgM and IgG antibodies against hCoV-EMC and SARS-CoV.
The assay showed minimal cross-reactivity with sera from individuals exposed to common hCoVs.
High specificity was confirmed using convalescent sera from confirmed hCoV-EMC and SARS-CoV infections.

Conclusions:

A novel and specific protein microarray assay for detecting hCoV-EMC and SARS-CoV antibodies has been successfully developed.
The assay provides a reliable tool for distinguishing between infections caused by emerging and known human coronaviruses.
This technology holds promise for improved diagnostics and epidemiological studies of coronavirus outbreaks.