Related Experiment Video

Updated: May 12, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

MicroRNA-23b inhibits enterovirus 71 replication through downregulation of EV71 VPl protein

Bai-ping Wen1, Hong-jian Dai, Yue-huang Yang

  • 1Kunming Children's Hospital, Kunming, China.

Intervirology
|April 19, 2013
PubMed

Insights

MicroRNAs (miRNAs), specifically hsa-miR-23b, show potential in combating Enterovirus 71 (EV71) infection. Upregulating miR-23b was found to inhibit EV71 replication, offering new avenues for treating hand-foot-and-mouth disease.

Area of Science:

  • Virology
  • Molecular Biology
  • Gene Regulation

Background:

  • Enterovirus 71 (EV71) causes hand-foot-and-mouth disease, with no current effective antiviral treatments.
  • MicroRNAs (miRNAs) regulate gene expression and are implicated in virus-host interactions.
  • Previous studies indicated hsa-miR-23b downregulation in EV71-infected cells.

Purpose of the Study:

  • To investigate the role of hsa-miR-23b in EV71 infection.
  • To determine if hsa-miR-23b can inhibit EV71 replication.
  • To identify the mechanism by which hsa-miR-23b affects EV71.

Main Methods:

  • Bioinformatic prediction of miR-23b target sites in the EV71 genome using miRanda.
  • Luciferase assays to confirm predicted target interactions.
  • Transfection of miR-23b mimics to assess its effect on EV71 replication.

Main Results:

  • hsa-miR-23b was predicted to target a conserved sequence in the EV71 3' untranslated region (UTR).
  • Upregulation of miR-23b significantly inhibited EV71 replication.
  • miR-23b inhibited EV71 replication by downregulating the EV71 VPl protein.

Conclusions:

  • hsa-miR-23b acts as a negative regulator of EV71 replication.
  • Targeting EV71's 3'UTR with miR-23b offers a potential therapeutic strategy.
  • This research enhances understanding for developing treatments against EV71-induced hand-foot-and-mouth disease.

More Related Videos

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus13:48

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Published on: June 24, 2012

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...