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Updated: May 12, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
Preparation of chitosan-graft-(β-cyclodextrin) based sol-gel stationary phase for open-tubular capillary
Haixia Lü1, Qingyin Li, Xiaowei Yu
1College of Materials Science and Engineering, Fuzhou University, Fuzhou, P. R. China. hx_lv@163.com
Abstract:
A novel open-tubular CEC column coated with chitosan-graft-(β-CD) (CDCS) was prepared using sol-gel technique. In the sol-gel approach, owing to the 3D network of sol-gel and the strong chemical bond between the stationary phase and the surface of capillary columns, good chromatographic characteristics and unique selectivity in separating isomers were shown. The column efficiencies of 55,000∼163,000 plates/m for the isomeric xanthopterin and phenoxy acid herbicides using the sol-gel-derived CDCS columns were achieved. Good stabilities were demonstrated that the RSD values for the retention time of thiourea and isoxanthopterin were 1.3 and 1.4% (run to run, n = 5), 1.6 and 2.0% (day to day, n = 3), 2.9 and 3.1% (column to column, n = 3), respectively. The sol-gel-coated CDCS columns have shown improved separations of isomeric xanthopterin in comparison with CDCS-bonded capillary column.
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